TRIS
- 77-86-1
- C4H11NO3
Tris or Tris(hydroxymethyl)aminomethane or THAM is an organic compound with the formula (HOCH2)3CNH2. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes.
| Tromethamine | |
| CAS: 77-86-1 | HS CODE: 2922199090 |
| Formula: C4H11NO3 | Molecular Weight: 121.14 |
| Structural Formula |
|
| Synonyms | TRIS BUFFER tris base Trometamol Tris-Amino |
| USAGE | |
Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris are used for protein crystal growth under different pH conditions. the low ionic strength characteristics of [3]Tris buffer can be used for the formation of intermediate fibers of c. elegans (C.elegans) nuclear fibrillar) protein. [4]Tris is also a major component of protein electrophoresis buffer. furthermore, Tris is the intermediate for the preparation of surfactants, vulcanizing promoters and some drugs. Tris is also used as a titration standard. | |
| SPECIFICATION | |
| Test items | Specifications |
| Appearance | white crystal |
| PURITY | 99.50%-101.0% |
| Melting point | 168.0-171.0℃ |
| Insolubles | ≤0.03% |
| ASH | ≤0.05% |
| Heavy metals | ≤5PPM |
| Loss on drying | ≤0.5% |
| PH | 10.0-11.5 |
| (Colour) | ≤5# |
| Fe | ≤5PPM |
| SO42+ | ≤10PPM |
| Cl | ≤10PPM |
| PROPERTIES | |
| Boiling Point: | 219-220 °C10 mm Hg(lit.) |
| Flash Point: | 219-220°C/10mm |
| Water-solubility: | 550 g/L (25 ºC) |
| Stability: | Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture. |
| Storage Condition: | |
| MSDS | TDS |
